Chromatin Immunoprecipitation:Article Title: 2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PSA sc-7638 Santa Cruz Biotechnology - 1/1000 dilution AR sc-816 Santa Cruz Biotechnology - 1/1000 dilution HSC70 sc-7298 Santa Cruz Biotechnology - 1/1000 dilution FKBP5 #12210 Cell Signaling Technology - 1/1000 dilution ACC #3676 Cell Signaling Technology - 1/1000 dilution ACLY #4332 Cell Signaling Technology - 1/1000 dilution p-ACLY #4331 Cell Signaling Technology - 1/1000 dilution PKM1/2 #3190 Cell Signaling Technology - 1/1000 dilution GLUT1 #12939 Cell Signaling Technology - 1/1000 dilution 3 nature research | reporting sum m ary O ctober 2018 AMPKa #2603 Cell Signaling Technology - 1/1000 dilution p-AMPKa #2535 Cell Signaling Technology - 1/1000 dilution CPT1a #12252 Cell Signaling Technology - 1/1000 dilution BIP #3177 Cell Signaling Technology - 1/1000 dilution DNAJC3 #2940 Cell Signaling Technology - 1/1000 dilution XBP1s #40435 Cell Signaling Technology - 1/1000 dilution CHOP #2895 Cell Signaling Technology - 1/1000 dilution EPCAM #93790 Cell Signaling Technology - 1/1000 dilution ATF4 #11815 Cell Signaling Technology - 1/1000 dilution PARP #9532 Cell Signaling Technology - 1/1000 dilution DECR1 ab95965 Abcam - 1/1000 dilution GPX4 ab125066 Abcam - 1/1000 dilution AR ChIP grade #5153 Cell Signaling Technology - 1ug/4ug chromatin AR ChIP grade 17-10489 Millipore - 1ug/4ug chromatin EnVision detection system K500711-2 Agilent - 1/1 dilution Goat anti-rabbit Alexa488 ab150077 abcam - 1/3000 dilution Anti-rabbit IgG HRP-linked #7074 Cell Signaling Technology - 1/3000 dilution Anti-mouse IgG HRP-linked #7076 Cell Signaling Technology - 1/3000 dilution Validation PSA sc-7638 Santa Cruz Biotechnology - validated for WB in βME-treated human prostate tissue extract (scbt website) AR sc-816 Santa Cruz Biotechnology - validated for WB using untreated and Brefeldin A treated LNCaP whole cell lysates (scbt website) HSC70 sc-7298 Santa Cruz Biotechnology - validated by WB using K-562, Hep G2, HCT-116, 3T3-L1, MCF7 and C6 whole cell lysates (scbt website).
Flow Cytometry:Article Title: 2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PSA sc-7638 Santa Cruz Biotechnology - 1/1000 dilution AR sc-816 Santa Cruz Biotechnology - 1/1000 dilution HSC70 sc-7298 Santa Cruz Biotechnology - 1/1000 dilution FKBP5 #12210 Cell Signaling Technology - 1/1000 dilution ACC #3676 Cell Signaling Technology - 1/1000 dilution ACLY #4332 Cell Signaling Technology - 1/1000 dilution p-ACLY #4331 Cell Signaling Technology - 1/1000 dilution PKM1/2 #3190 Cell Signaling Technology - 1/1000 dilution GLUT1 #12939 Cell Signaling Technology - 1/1000 dilution 3 nature research | reporting sum m ary O ctober 2018 AMPKa #2603 Cell Signaling Technology - 1/1000 dilution p-AMPKa #2535 Cell Signaling Technology - 1/1000 dilution CPT1a #12252 Cell Signaling Technology - 1/1000 dilution BIP #3177 Cell Signaling Technology - 1/1000 dilution DNAJC3 #2940 Cell Signaling Technology - 1/1000 dilution XBP1s #40435 Cell Signaling Technology - 1/1000 dilution CHOP #2895 Cell Signaling Technology - 1/1000 dilution EPCAM #93790 Cell Signaling Technology - 1/1000 dilution ATF4 #11815 Cell Signaling Technology - 1/1000 dilution PARP #9532 Cell Signaling Technology - 1/1000 dilution DECR1 ab95965 Abcam - 1/1000 dilution GPX4 ab125066 Abcam - 1/1000 dilution AR ChIP grade #5153 Cell Signaling Technology - 1ug/4ug chromatin AR ChIP grade 17-10489 Millipore - 1ug/4ug chromatin EnVision detection system K500711-2 Agilent - 1/1 dilution Goat anti-rabbit Alexa488 ab150077 abcam - 1/3000 dilution Anti-rabbit IgG HRP-linked #7074 Cell Signaling Technology - 1/3000 dilution Anti-mouse IgG HRP-linked #7076 Cell Signaling Technology - 1/3000 dilution Validation PSA sc-7638 Santa Cruz Biotechnology - validated for WB in βME-treated human prostate tissue extract (scbt website) AR sc-816 Santa Cruz Biotechnology - validated for WB using untreated and Brefeldin A treated LNCaP whole cell lysates (scbt website) HSC70 sc-7298 Santa Cruz Biotechnology - validated by WB using K-562, Hep G2, HCT-116, 3T3-L1, MCF7 and C6 whole cell lysates (scbt website).
Magnetic Resonance Imaging:Article Title: 2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PSA sc-7638 Santa Cruz Biotechnology - 1/1000 dilution AR sc-816 Santa Cruz Biotechnology - 1/1000 dilution HSC70 sc-7298 Santa Cruz Biotechnology - 1/1000 dilution FKBP5 #12210 Cell Signaling Technology - 1/1000 dilution ACC #3676 Cell Signaling Technology - 1/1000 dilution ACLY #4332 Cell Signaling Technology - 1/1000 dilution p-ACLY #4331 Cell Signaling Technology - 1/1000 dilution PKM1/2 #3190 Cell Signaling Technology - 1/1000 dilution GLUT1 #12939 Cell Signaling Technology - 1/1000 dilution 3 nature research | reporting sum m ary O ctober 2018 AMPKa #2603 Cell Signaling Technology - 1/1000 dilution p-AMPKa #2535 Cell Signaling Technology - 1/1000 dilution CPT1a #12252 Cell Signaling Technology - 1/1000 dilution BIP #3177 Cell Signaling Technology - 1/1000 dilution DNAJC3 #2940 Cell Signaling Technology - 1/1000 dilution XBP1s #40435 Cell Signaling Technology - 1/1000 dilution CHOP #2895 Cell Signaling Technology - 1/1000 dilution EPCAM #93790 Cell Signaling Technology - 1/1000 dilution ATF4 #11815 Cell Signaling Technology - 1/1000 dilution PARP #9532 Cell Signaling Technology - 1/1000 dilution DECR1 ab95965 Abcam - 1/1000 dilution GPX4 ab125066 Abcam - 1/1000 dilution AR ChIP grade #5153 Cell Signaling Technology - 1ug/4ug chromatin AR ChIP grade 17-10489 Millipore - 1ug/4ug chromatin EnVision detection system K500711-2 Agilent - 1/1 dilution Goat anti-rabbit Alexa488 ab150077 abcam - 1/3000 dilution Anti-rabbit IgG HRP-linked #7074 Cell Signaling Technology - 1/3000 dilution Anti-mouse IgG HRP-linked #7076 Cell Signaling Technology - 1/3000 dilution Validation PSA sc-7638 Santa Cruz Biotechnology - validated for WB in βME-treated human prostate tissue extract (scbt website) AR sc-816 Santa Cruz Biotechnology - validated for WB using untreated and Brefeldin A treated LNCaP whole cell lysates (scbt website) HSC70 sc-7298 Santa Cruz Biotechnology - validated by WB using K-562, Hep G2, HCT-116, 3T3-L1, MCF7 and C6 whole cell lysates (scbt website).
Biomarker Discovery:Article Title: 2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PSA sc-7638 Santa Cruz Biotechnology - 1/1000 dilution AR sc-816 Santa Cruz Biotechnology - 1/1000 dilution HSC70 sc-7298 Santa Cruz Biotechnology - 1/1000 dilution FKBP5 #12210 Cell Signaling Technology - 1/1000 dilution ACC #3676 Cell Signaling Technology - 1/1000 dilution ACLY #4332 Cell Signaling Technology - 1/1000 dilution p-ACLY #4331 Cell Signaling Technology - 1/1000 dilution PKM1/2 #3190 Cell Signaling Technology - 1/1000 dilution GLUT1 #12939 Cell Signaling Technology - 1/1000 dilution 3 nature research | reporting sum m ary O ctober 2018 AMPKa #2603 Cell Signaling Technology - 1/1000 dilution p-AMPKa #2535 Cell Signaling Technology - 1/1000 dilution CPT1a #12252 Cell Signaling Technology - 1/1000 dilution BIP #3177 Cell Signaling Technology - 1/1000 dilution DNAJC3 #2940 Cell Signaling Technology - 1/1000 dilution XBP1s #40435 Cell Signaling Technology - 1/1000 dilution CHOP #2895 Cell Signaling Technology - 1/1000 dilution EPCAM #93790 Cell Signaling Technology - 1/1000 dilution ATF4 #11815 Cell Signaling Technology - 1/1000 dilution PARP #9532 Cell Signaling Technology - 1/1000 dilution DECR1 ab95965 Abcam - 1/1000 dilution GPX4 ab125066 Abcam - 1/1000 dilution AR ChIP grade #5153 Cell Signaling Technology - 1ug/4ug chromatin AR ChIP grade 17-10489 Millipore - 1ug/4ug chromatin EnVision detection system K500711-2 Agilent - 1/1 dilution Goat anti-rabbit Alexa488 ab150077 abcam - 1/3000 dilution Anti-rabbit IgG HRP-linked #7074 Cell Signaling Technology - 1/3000 dilution Anti-mouse IgG HRP-linked #7076 Cell Signaling Technology - 1/3000 dilution Validation PSA sc-7638 Santa Cruz Biotechnology - validated for WB in βME-treated human prostate tissue extract (scbt website) AR sc-816 Santa Cruz Biotechnology - validated for WB using untreated and Brefeldin A treated LNCaP whole cell lysates (scbt website) HSC70 sc-7298 Santa Cruz Biotechnology - validated by WB using K-562, Hep G2, HCT-116, 3T3-L1, MCF7 and C6 whole cell lysates (scbt website).
Western Blot:Article Title: 2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PSA sc-7638 Santa Cruz Biotechnology - 1/1000 dilution AR sc-816 Santa Cruz Biotechnology - 1/1000 dilution HSC70 sc-7298 Santa Cruz Biotechnology - 1/1000 dilution FKBP5 #12210 Cell Signaling Technology - 1/1000 dilution ACC #3676 Cell Signaling Technology - 1/1000 dilution ACLY #4332 Cell Signaling Technology - 1/1000 dilution p-ACLY #4331 Cell Signaling Technology - 1/1000 dilution PKM1/2 #3190 Cell Signaling Technology - 1/1000 dilution GLUT1 #12939 Cell Signaling Technology - 1/1000 dilution 3 nature research | reporting sum m ary O ctober 2018 AMPKa #2603 Cell Signaling Technology - 1/1000 dilution p-AMPKa #2535 Cell Signaling Technology - 1/1000 dilution CPT1a #12252 Cell Signaling Technology - 1/1000 dilution BIP #3177 Cell Signaling Technology - 1/1000 dilution DNAJC3 #2940 Cell Signaling Technology - 1/1000 dilution XBP1s #40435 Cell Signaling Technology - 1/1000 dilution CHOP #2895 Cell Signaling Technology - 1/1000 dilution EPCAM #93790 Cell Signaling Technology - 1/1000 dilution ATF4 #11815 Cell Signaling Technology - 1/1000 dilution PARP #9532 Cell Signaling Technology - 1/1000 dilution DECR1 ab95965 Abcam - 1/1000 dilution GPX4 ab125066 Abcam - 1/1000 dilution AR ChIP grade #5153 Cell Signaling Technology - 1ug/4ug chromatin AR ChIP grade 17-10489 Millipore - 1ug/4ug chromatin EnVision detection system K500711-2 Agilent - 1/1 dilution Goat anti-rabbit Alexa488 ab150077 abcam - 1/3000 dilution Anti-rabbit IgG HRP-linked #7074 Cell Signaling Technology - 1/3000 dilution Anti-mouse IgG HRP-linked #7076 Cell Signaling Technology - 1/3000 dilution Validation PSA sc-7638 Santa Cruz Biotechnology - validated for WB in βME-treated human prostate tissue extract (scbt website) AR sc-816 Santa Cruz Biotechnology - validated for WB using untreated and Brefeldin A treated LNCaP whole cell lysates (scbt website) HSC70 sc-7298 Santa Cruz Biotechnology - validated by WB using K-562, Hep G2, HCT-116, 3T3-L1, MCF7 and C6 whole cell lysates (scbt website).
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